دانشگاه علوم پزشکی جندی شاپور اهواز مجله علمی پزشکی جندی شاپور 2252-052X 16 2 2017 05 22 The Effect of Deprivation of Food and Water on Formation of Urinary Crystals in Rat’s Kidney as an Experimental Model بررسی تأثیر محرومیت از آب و غذا بر تشکیل کریستال ادراری در کلیه موش صحرایی به عنوان یک مدل تجربی 185 195 49249 10.22118/jsmj.2017.49249 FA مهزیار خزاعلی استادیار گروه اورولوژی.گروه اورولوژی، بیمارستان امام خمینی، دانشگاه علوم پزشکی جندی‌شاپور اهواز، اهواز، ایران. سجاد نیکخواه دستیار تخصصی اورولوژی.گروه اورولوژی، بیمارستان امام خمینی، دانشگاه علوم پزشکی جندی‌شاپور اهواز، اهواز، ایران. سید مختار حسینی استادیار گروه اورولوژی.گروه اورولوژی، بیمارستان امام خمینی، دانشگاه علوم پزشکی جندی‌شاپور اهواز، اهواز، ایران. احمد روحی زاده دکترای تخصصی کلینیکال پاتولوژی.مرکز تحقیقات سلولی و مولکولی، دانشگاه علوم پزشکی جندی‌شاپور اهواز، اهواز، ایران. Journal Article 2017 03 02 Backgroundand Objective:During the holy month of Ramadan, the number of admissions of patients with renal colic increases. This study was conducted to investigate the effects deprivation of food and water on the urinary crystals formation in the kidneys of rats as an experimental model of Ramadan-type fasting (RTF). Subjects and Methods: In this experimental study, 32 male Wistar rats were randomly allocated into four groups. The first (Ct) and the third (EG) groups of the rats received standard pellet diet and water ad libitum. The second (RTF) and the fourth (RTF+EG) groups of the animals were fasted (12 h) and then re-fed (12 h) daily. Drinking water containing 0.75% ethylene glycol were given to EG and RTF+EG groups. The existence and the frequency of crystal deposition of calcium oxalate in the renal tissue stained by Hematoxylin and Eosin (HE) were observed in each group by light microscopy. Frequency of crystal deposition on 10 fields (×20) was calculated for each group. The results are expressed as Mean±SEM and also was graded semi quantitatively in five categories as (-), (+), (++), (+++), and (++++), respectively. Results: Oxalate calcium crystals were not observed in Ct and RTF groups. The Mean±SEM of crystal deposition in the EG and RTF+EG groups were 4.9±0.29 and 4.2±0.24, respectively. There was no statistically significant difference between the two groups. Conclusions: The results of this study showed that there are not significant associations between deprivation of food and water with formation as well as amount of calcium oxalate deposition in kidney. Backgroundand Objective:During the holy month of Ramadan, the number of admissions of patients with renal colic increases. This study was conducted to investigate the effects deprivation of food and water on the urinary crystals formation in the kidneys of rats as an experimental model of Ramadan-type fasting (RTF). Subjects and Methods: In this experimental study, 32 male Wistar rats were randomly allocated into four groups. The first (Ct) and the third (EG) groups of the rats received standard pellet diet and water ad libitum. The second (RTF) and the fourth (RTF+EG) groups of the animals were fasted (12 h) and then re-fed (12 h) daily. Drinking water containing 0.75% ethylene glycol were given to EG and RTF+EG groups. The existence and the frequency of crystal deposition of calcium oxalate in the renal tissue stained by Hematoxylin and Eosin (HE) were observed in each group by light microscopy. Frequency of crystal deposition on 10 fields (×20) was calculated for each group. The results are expressed as Mean±SEM and also was graded semi quantitatively in five categories as (-), (+), (++), (+++), and (++++), respectively. Results: Oxalate calcium crystals were not observed in Ct and RTF groups. The Mean±SEM of crystal deposition in the EG and RTF+EG groups were 4.9±0.29 and 4.2±0.24, respectively. There was no statistically significant difference between the two groups. Conclusions: The results of this study showed that there are not significant associations between deprivation of food and water with formation as well as amount of calcium oxalate deposition in kidney.