بررسی نقش گیرنده نوع 3- سروتونین در ناحیه CA1 هیپوکامپ مغز بر فراموشی ناشی از محرومیت 6 ساعته از خواب متناقض RSD) REM) و خواب کلی(TSD)

نوع مقاله: مقاله پژوهشی

نویسنده

مدرس-زیست شناسی-دانشگاه پیام نور-آموزش و پرورش

10.22118/jsmj.2018.59642

چکیده

زمینه و هدف: خواب یک حالت برگشت پذیر است که ظرفیت واکنش و تعامل با محیط را محدود می کند. گیرنده 5HT3 که بطور گسترده در ناحیه CA1 هیپوکامپ مغز بیان می شود، نقشی حیاتی در تنظیم خواب و تثبیت حافظه دارد. هدف این مطالعه بررسی تاثیر فعال سازی و غیرفعال سازی گیرنده 5HT3 به ترتیب با Y25130 و MCHL بر اختلال تثبیت حافظه تحت القای محرومیت از دو نوع خواب کلی total sleep deprivation)) وخواب متناقض REM sleep deprivation) ) در موش صحرایی بود.
روش بررسی: موشهای صحرایی نر نژاد ویستار بطور تصادفی درپنج گروه هشت تایی قرارگرفتند:1 –دو گروه دریافت کننده دوزهای موثر آگونیست و آنتاگونیست گیرنده 5HT3 2-  شاهد TSD، 3-با محرومیت از خواب TSD 4- شاهد RSD 5-با محرومیت از خواب .RSD
 از تکنیک چندسکویی برای القای RSD و تکنیک واتر باکس برای القای TSD و آزمون اجتناب غیرفعال برای ارزیابی تثبیت حافظه استفاده شده است.
یافته ها:  دوزهای 01/0 و 001/0 میلی گرم آگونیست گیرنده 5HT3  (MCHL) و دوز 1/0 میلی گرم آنتاگونیست گیرنده 5HT3 (Y25130)  بدون محرومیت از خواب باعث کاهش معنی دار حافظه اجتنابی  (P<0.01)شد. در حالی که MCHL با دوز1/0 و نیز Y25130 با دوزهای 1/0 و 01/0میلی گرم بطور معنی داری فعالیت حرکتی را افزایش دادند (P<0.01)، اما هر دو دارو درتمامی دوزها بر روی درد تاثیر معنی داری نداشتند. دوز زیر آستانه  Y25130 فراموشی ناشی از TSD و RSD را بطور معنی داری برگرداند (P<0.01). در حالی که هر دو دارو در شرایط TSD و RSD تاثیر معنی داری بر فعالیت حرکتی را نشان ندادند. اما MCHL  در شرایط RSD  و Y25130  در شرایط TSD بطور معنی داری بی دردی را افزایش دادند (P<0.01).
نتیجه گیری: بر اساس یافته های این تحقیق بنظر میرسد گیرنده های 5HT3 در ناحیهCA1 هیپوکامپ نقش حیاتی در رفتارهای شناختی و غیر شناختی ایجاد شده بوسیله TSD و RSD بازی می کنند.

کلیدواژه‌ها


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