The effect of regular aerobic training and melatonin consumption on the activity of NF-κB and Nrf2 genes and oxidant factors in the heart tissue of male desert rats model of type 1 diabetes

Articles in Press

Document Type : Original Article

Authors

1 Department of Physical Education & Sport Sciences, Islamic Azad University, Ahvaz Branch. Ahvaz, Iran

2 Department of Physical Education & Sport Sciences, Ahvaz Branch, Islamic Azad University, Ahvaz, Iran

10.22118/jsmj.2024.428804.3317

Abstract

Background and Objectives: Research has shown that NF-κB, Nrf2 genes are the regulators of oxidative stress. The purpose of this research was the effect of regular aerobic exercise and melatonin consumption on the activity of NF-κB and Nrf2 genes and oxidant factors in the heart tissue of type 1 diabetes male field rats in the heart tissue of diabetic male rats.

Subjects and Methods: 40 male Wistar rats were randomly divided into 5 experimental groups including diabetes, 2- diabetes+melatonin, 3- diabetes+exercise, 4- diabetes+melatonin+exercise and 5- healthy control. The diabetes model was induced by injecting 50 mg/kg of streptozotocin. The exercise groups performed 42 days of forced aerobic exercise (intensity: 15 m/min; 30 min) using a rodent treadmill. Also, in the melatonin administration groups, rats received 10 mg/kg melatonin (intraperitoneal) for 42 days. After sampling from heart tissue, NF-κB and Nrf2 genes were measured by real time PCR method.

Results: Exercise along with melatonin administration caused a significant decrease in NF-κB gene expression. On the other hand, the level of Nrf2 gene expression increased in the treatment groups compared to the diabetic group. Also, the level of MDA in the heart tissue of desert rats was significantly reduced after melatonin exercise. But the level of TAC in the heart tissue of the treatment group increased compared to the diabetic rats.

Conclusion: The results showed that exercise along with melatonin supplement can improve heart damage caused by diabetes by reducing oxidative stress and heart tissue inflammation.

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Jundishapur Scientific Medical Journal

Articles in Press, Accepted Manuscript
Available Online from 08 May 2024

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History

  • Receive Date: 04 December 2023
  • Revise Date: 01 May 2024
  • Accept Date: 08 May 2024

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