The Impact of BCL11A and HBS1L-MYB Polymorphic Markers in Affected and Carriers of Beta Thalassemia with High Level of Fetal Hemoglobin in South of Iran, Khuzestan
Gholamreza
Shariati
Narges Medical Genetics & PND Laboratory, Ahvaz ,Iran
author
Mohammad
Hamid
Assistant Professor of Molecular Medicine.Department of Molecular Medicine, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran.
author
Nilofar
Barkhordari Bafghi
Master of Science in Molecular Medicine.Department of Molecular Medicine, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran.
author
Hamid
Galehdari
Professor of Genetic.Department of Medical Genetic, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
author
Ali Hossein
Saberi
Associate Professor of Genetic.Department of Medical Genetic, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
author
Ali Reza
Sedaghat
Associate Professor of Endocrinology.
Laboratory of Genetic Medicine and Prenatal Diagnosis, Narges, Ahvaz, Iran.
author
text
article
2018
per
Background and Objective: The increase of HbF in some beta-thalassemia and sickle cell patients ameliorates the disease severity. Therefore, understanding the reasons behind the increasing of fetal hemoglobin is needful. The molecular explanation of fetal hemoglobin increment might be correlated with the genes which play a regulatory role in expression of beta-globin cluster genes. In this study, the likelihood of polymorphisms of BCL11A and HBS1L-MYB regions to cause HbFelevation was studied. Subjects and Methods: In this investigation, 50 thalassemia patients with high level of fetal hemoglobin and 50 healthy individuals as control group were selected from Khuzestan. The allele frequency of rs28384513, rs766432, rs11886868 and rs4895441 polymorphisms were assessed by using PCR-RFLP and PCR- sequencing methods. Results: The frequency of C allele in rs766432 (A/C), C allele in rs11886868 (C/T) and A allele in rs28384513 (A/C) were significantly high among the patients with increased level of HbF. Whereas, the frequency of rs4895441 (A/G) polymorphism had no significant differences compared with the patients with high level of HbF and control group. Conclusion: Based on this study, rs766432 (A/C), rs11886868 (C/T) and rs28384513 (A/C) polymorphisms are correlated with HbF increased and they can be used to predict the clinical features of fetus in case of bearing the major or intermediate forms of thalassemia. However, to confirm these results these markers need to be studied by applying a larger number of samples.
Jundishapur Scientific Medical Journal
Ahvaz Jundishapur University of Medical Sciences
2252-052X
16
v.
6
no.
2018
611
619
https://jsmj.ajums.ac.ir/article_57806_3d8ca067ac5873bf0f2c1dde115199d2.pdf
dx.doi.org/10.22118/jsmj.2018.57806
Recombinant Expression of Staphylococcus aureus Enterotoxin Type B as a Vaccine Candidate
Seyed Amir
Hosseini
PhD Student in Nanobiotechnology.Biology Research Center, Faculty of Basic Science, Imam Hossein University, Tehran, Iran.
author
Firouz
Ebrahimi
Assistant Professor of Biology.Department of Biology, Faculty of Basic Science, Imam Hossein University, Tehran, Iran.
author
Shahram
Nazarian
Assistant Professor of Biology.Department of Biology, Faculty of Basic Science, Imam Hossein University, Tehran, Iran.
author
Mahmud
Hamidi
MSc Student in Cellular and Molecular biology.Biology Research Center, Faculty of Basic Science, Imam Hossein University, Tehran, Iran.
author
text
article
2018
per
Background and Objective: Staphylococcus aureus is a gram-positive cocci that causes many diseases, such as food poisoning, risky shocks. Entrotoxins are the most important toxins of the bacteria. Among them, enterotoxin type B is the most common and important ones which is a super antigen. The aim of this study was the cloning and recombinant expression of seb gene in order to achieve a stable construct for the protein production and further use as a vaccine candidate in future investigations. Subjects and Methods: seb gene was analyzed for rare codons and gene optimaztion was performed using bioinformatic softwares. Enzymatic digestion was performed to confirm the presence of the seb gene into pET28a(+) expression vector. Recombinant expression of SEB was induced by IPTG following cloning confirmation. Then, protein purification was done under non-denaturing conditions using a nickel chromatography column. Protein confirmation was performed by Western blotting analysis. Results: Codon adaptation index (CAI) of the native gene was 0.68, while the optimized sequence had a CAI of 0.82.Percentage of codon having high frequency distribution was improved to 57%. Restriction analysis confirmed the cloning of the seb gene into pET28a vector. The results showed that the cloning of seb gene in pET28a(+) vector was performed in an appropriate position between expected restriction sites. In addition, SEB had a good and significant expression after induction by IPTG. The total yield of purified protein was 22mg/L. Western blotting showed specific reactivity of recombinant protein with anti-his tag antibody. Conclusion: Stable recombinant construct containing seb gene was constructed and appropriate recombinant expression of the protein was observed. So, this protein could be used in future studies as a vaccine candidate against SEB toxin of Staphylococcus aureus.
Jundishapur Scientific Medical Journal
Ahvaz Jundishapur University of Medical Sciences
2252-052X
16
v.
6
no.
2018
653
664
https://jsmj.ajums.ac.ir/article_57807_c9d5fe19e5aabcb67d2444f906e979a7.pdf
dx.doi.org/10.22118/jsmj.2018.57807
Effects of Aqueous Cinnamon Extract on Body Mass Index, Organs Weight and Biochemical Indices of Male Wistar
Rats on a High-fat Diet
Somayeh
Rostamkhani
MSc of Animal Physiology.Department of Animal Physiology, Science and Research Branch of Islamic Azad University of Zanjan, Zanjan, Iran.
author
Sanaz
Mahmazi
Assistant Professor of Genetics.Department of Genetics, Biology Research Center of Islamic Azad University of Zanjan, Zanjan, Iran.
author
Mehrdad
Ghorbanlou
MSc of Anatomical Sciences.Department of Anatomical Sciences, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran
author
Saeed
Shokri
Associate Professor of Anatomical Sciences.Department of Anatomical Sciences, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran
author
Vahid
Bayati
Assistant Professor of Anatomical Sciences.
Department of Anatomical Sciences, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
author
Reza
Nejatbakhsh
Associate Professor of Anatomical Sciences.Department of Anatomical Sciences, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran
author
text
article
2018
per
Background and Objective: Consuming herbal supplements for preventing obesity is cheaper and safer in comparison to chemical drugs. Considering the anti-obesity effect of Cinnamon, in this study, effects of two different doses of aqueous Cinnamon extract on weight and biochemical indices related to metabolic syndrome were investigated in rats under high-fat diet. Subjects and Methods: Thirty two male Wistar rats were divided into four groups:Control (normal diet), high-fat diet group (high-fat diet with 60% additional fat), 2 high-fat diet groups treated with 100 mg/kg and 200 mg/kg of extract. Treatment was done daily for 20 weeks by gavage. Then, dissection was performed and blood samples were taken from the heart. Weights of internal organs and omental fat were measured, and biochemical tests were performed. Results: One hundred mg/kg dose of extract, reduced BMI and omental fat in comparison to the high-fat diet group. Both doses of extract reduced leptin level in comparison to the high-fat diet group. Dose of 100 mg/kg prevented the weight reduction of pancreas. Conclusion: In the present study, anti-obesity effects of 100 mg/kg dose of extract was observed which may be an evidence of positive effects of low doses of extract. Aqueous Cinnamon extract can be used as a flavor with meals.
Jundishapur Scientific Medical Journal
Ahvaz Jundishapur University of Medical Sciences
2252-052X
16
v.
6
no.
2018
693
703
https://jsmj.ajums.ac.ir/article_57809_79e5dca9d56d3fac9a9b0addd8b574b1.pdf
dx.doi.org/10.22118/jsmj.2018.57809
Effect of Eight Weeks of Swimming Training with Garlic Intake on Serum Levels of IL-10 and TNF-α in Obese Male Rats
Davood
Farazandeh Nia
Master of Sport Physiology.Department of Exercise physiology, East Tehran Branch, Islamic Azad University, Tehran, Iran.
author
Masoumeh
Hosseini
member of scientific board Islamic Azad Uni East Tehran Branch
author
Shahin
Riyahi Malayeri
Assistant Professor of Sport Physiology.Department of Exercise physiology, East Tehran Branch, Islamic Azad University, Tehran, Iran.
author
Abdolrasoul
Daneshjoo
Assistant Professor of Sport Biomechanics.Department of exercise Biomechanics, East Tehran Branch, Islamic Azad University, Tehran, Iran.
author
text
article
2018
per
Background and Objective: Tumor necrosis factor alpha (TNF-α) as a pro inflammatory cytokine and interlokin-10 as an anti-inflammatory cytokine have an important role in the development and the prevention of the systemic inflammation and incidence of obesity-induced diseases such as Coronary artery disease respectively .The purpose of this study was determining the effect of eight weeks of swimming training with garlic intake on serum levels of IL-10 and TNF-α in obese male rats. Subjects and Methods: During this experiment 28 Wister male rats with obesity) 250-300 grams (were divided into four groups (control, supplement, training, training + supplement) randomly. The training groups swam for 8 weeks, 3 sessions per week and 60 minutes each session. The supplement groups received a solution of garlic extract in dose of 1 milliliter per kilogram of body weight for 8 weeks. Blood samples were taken 48 h after the last training session and the levels of IL-10 and TNF-α were measured. Statistical methods: One-way ANOVA were used for data analysis. Results: The results showed that eight weeks training and garlic consumption significantly increased the levels of IL-10 and significantly decreased TNF-α in experimental groups (P<0.001). No significant correlations were found between the levels of IL-10 and TNF-α (P>0.05). Conclusion: Implementing 8 week of swimming training with garlic intake may have a positive effect on inflammatory factors by increasing IL-10 and decreasing TNF-α.
Jundishapur Scientific Medical Journal
Ahvaz Jundishapur University of Medical Sciences
2252-052X
16
v.
6
no.
2018
665
671
https://jsmj.ajums.ac.ir/article_57830_112450dacbdea43846f728c0e1f83718.pdf
dx.doi.org/10.22118/jsmj.2018.57830
Study of the Contamination Rate of Vegetables by Enterohemorrhagic Escherichia coli (EHEC) Using Multiplex PCR and Cultivation Methods in Ahvaz Province
Setareh
Shams Solari
M.Sc Student in Microbiology. Department of Microbiology, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
author
Mohammad
Roayaei Ardekani
Ph.D in Microbiology.Department of Microbiology, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
author
Seyedeh Elham
Rezatofighi
Assistant Professor of Microbiology.Department of Microbiology, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
author
text
article
2018
per
Background and Objective: Enterohemorrhagic Escherichia coli is a pathogen that can be transmitted to humans through food products and causes dangerous diseases such as hemorrhagic colitis and hemolytic uremic syndrome in humans because of shiga toxin production. Materials and Method: In this research, 256 samples of fresh vegetables including lettuce, cabbage and eating herbs were prepared from 4 vegetable wholesale centers in the city of Ahvaz during 8 months. After primary enrichment, Sorbitol negative colonies grown on CT-SMAC medium were subcultured on differential culture media and confirmed as E. coli by specific biochemical tests. Confirmed strains were then investigated by E.coli O157:H7 antiserum. Finally, the presence of stx2, stx1, eaeA and hlyA genes was evaluated in sorbitol negative strains using multiplex PCR. Result: 8 E.coli O157 (3.1%) bacteria were detected, only 2 isolates were identified as E.coli O157:H7. After multiplex PCR it became clear that 4 isolates contained stx1 gene, 2 isolates stx2 gene, 1 isolate stx1 and eaeA genes, 1 isolate stx1, eaeA and hlyA genes and 1 isolate stx1 and hlyA genes. None of the isolates contained all four genes. Conclusion: The presence of E.coli bacteria in vegetables is problematic for public health and can cause food-borne diseases.
Jundishapur Scientific Medical Journal
Ahvaz Jundishapur University of Medical Sciences
2252-052X
16
v.
6
no.
2018
671
680
https://jsmj.ajums.ac.ir/article_57855_3bdc77188fb51015117d61aaac0cbda7.pdf
dx.doi.org/10.22118/jsmj.2018.57855
Effect of Aerobic Training on Blood Pressure, HbA1c and Arterial Stiffness in Postmenopausal Women with Type 2 Diabetes
Hamid
Rajabi
Associate Professor of Sport Physiology.Department of Sport Physiology, Faculty of Physical Education, Kharazmi University of Tehran, Tehran, Iran.
author
adel
donyai
Department of Exercise Physiology, Faculty of Sport Sciences, University of Kharazmi, Tehran, Iran
author
Pezhman
Motamedi
Assistant Professor of Sport Physiology.Department of Sport Physiology, Faculty of Physical Education, Kharazmi University of Tehran, Tehran, Iran.
author
Mohammad Reza
Dehkhoda
Associate Professor of Sport Physiology.Department of Sport Physiology, Faculty of Physical Education, Kharazmi University of Tehran, Tehran, Iran.
author
text
article
2018
per
Background and Objectives: More than 80% of diabetic subjects die of cardiovascular complications. One of the problems in diabetics is vascular arterial stiffness. The aim of present study was to investigate the effect of eight weeks aerobic training on blood pressure, HbA1c and arterial stiffness in postmenopausal women with type 2 diabetes. Subjects and Methods: Twenty-two diabetic women were randomly divided into the experimental (n=11, age: 51±6.2 yrs, BMI: 32.4±5) or the control (n=11, age: 52.3±5.6 yrs, BMI: 29.4±3.9) groups. The experimental group performed aerobic exercise program for 8 weeks at the intensity of 50–80% of maximum heart rate, 3 sessions per week. Blood samples were taken 24 h before and 48 h after the experimental period. Arterial stiffness evaluation under standard conditions was also performed using VaSera-VS-2000 (Fukuda Denshi Co., Japan). Results: Intra-group analysis showed that systolic blood pressure, HbA1C, CAVI index significantly decreased in the experimental group. The analysis of covariance also showed a significant difference between groups in CAVI index (P≤0.05). Conclusion: Overall, aerobic exercise could influence the factors affecting the compliance of vessels as well as factors related to the glycosylation, lowering blood pressure and indicators of arterial stifness (CAVI) and thus can be used as a suitable strategy in this regard.
Jundishapur Scientific Medical Journal
Ahvaz Jundishapur University of Medical Sciences
2252-052X
16
v.
6
no.
2018
631
642
https://jsmj.ajums.ac.ir/article_57901_72b75bba09133116620c648adeaa2f63.pdf
dx.doi.org/10.22118/jsmj.2018.57901
Effects of Progressive Dual-task Training on Motor Performance, Cognitive Status and Fall Risk among Older Adults With and Without Mild Cognitive Impairment
Faraz
PakZamir
PhD Candidate of Motor Behavior, Department of Motor Behavior. Motor Learning and Control Dep, Tehran, Iran.
author
Mehdi
Shahbazi
Associate Professor of Motor Behavior.
Department of Motor Behavior. Motor Learning and Control Dep, Tehran, Iran.
author
Fazlollah
Bagher Zadeh
Associate Professor of Motor Behavior. Department of Motor Behavior. Motor Learning and Control Dep, Tehran, Iran.
author
Elaheh
Arab Ameri
Associate Professor of Motor Behavior.Department of Motor Behavior. Motor Learning and Control Dep, Tehran, Iran.
author
text
article
2018
per
Background and Objective: Patients with Mild Cognitive Impairment (MCI) demonstrate inadequacy in compensatory techniques and lacking motor control while falling; hence it appears that cognitive and motor deficiencies are inter-related. The aim of this study was to investigate the progressive dual-task effects on motor performance, cognitive status and fall risk between older adults. Subjects and Methods: This study is semi experimental with pre– and post-test design. The subjects were selected from community dwelling elderly adults with mean age of 75.65 y (range, 65–90). Both groups (20 with MCI and 20 Healthy) participated in 12 weeks of intervention with progressive dual-task training, which entailed sitting, standing and transforming practices. Results: Examinations after 12 weeks showed that intervention caused improvements in cognitive status, postural control, and fall risk in both older adults with and without MCI. The MCI group experienced more positive changes in cognition, while the normal group had the most evident effects in fall risk and performance in Functional Reach (FR) test. Conclusion: This finding supports the previous results which implied the positive effects of dual-task training on cognitive variables are associated to biological variations which arise during exercise training. We also suggest that the physical benefits of progressive dual-task training (e.g. flexibility) are overriding the cognitive changes. In future research, the pathophysiology and further clinical implications should be investigated.
Jundishapur Scientific Medical Journal
Ahvaz Jundishapur University of Medical Sciences
2252-052X
16
v.
6
no.
2018
599
609
https://jsmj.ajums.ac.ir/article_57908_a5c5800b02ddae8cd1880cfdc2ad0cd8.pdf
dx.doi.org/10.22118/jsmj.2018.57908
Expression of Wnt and Notch Signaling Pathways are Increased in Cancer Stem-Like Cells in Metastatic Melanoma Cell Line (A375)
Maryam
MehdiPour
Master of Science in Animal Biology, Towards Cellular-Tactile Science.Department of Animal Biology, Tow-Transe Science, Science & Culture University, Tehran, Iran.
author
Faezeh
Keighobadi
Student MSc in Animal Biology. Towards Cellular-Tangential Sciences.Department of Animal Biology, Tow-Transe Science, Science & Culture University, Tehran, Iran.
author
Javad
Firouzi
Group of Stem Cells and Developmental Biology.Department of Stem Cells and Developmental Biology, Cellular Research Center, Stem Cell and Tactical Biology Department, Institute of Biology and Technology of Stem Cells, University of Tehran, Tehran, Iran.
author
Pardis
Khosravani
Senior Cell and Molecular Expert.Department of Stem Cells and Developmental Biology, Cellular Research Center, Stem Cell and Tactical Biology Department, Institute of Biology and Technology of Stem Cells, University of Tehran, Tehran, Iran.
author
Marzieh
Ebrahimi
Associate Professor Royan Research Group.Department of Stem Cells and Developmental Biology, Cellular Research Center, Stem Cell and Tactical Biology Department, Institute of Biology and Technology of Stem Cells, University of Tehran, Tehran, Iran.
author
text
article
2018
per
Backgound and Objective: Cancer stem cells assume to be responsible for increasing cancer properties such as migration, and drug sensitivity. A lot of studies revealed signaling pathways are enhanced in cancer stem cells. Melanoma is well-known as heterogeneous cancer and its severity in treatment. These can be attributed to the existence of cancer stem cells. In this study, we first aimed to separate cancer stem cells (cancer stem-like cells) by sphere formation, characterized them, and second examine the expression of Wnt and Notch signaling pathway genes related to adherent cells. Subjects and Methods: Adherent cell were cultured in the non-adherent condition with serum-free media; spheres obtained, then sphere formation, clonogenic assay, expression of CD133 and Nestin proteins, Nanog, NESTIN and Oct4 genes as stem related genes were assessed in comparison to adherent cells. In addition, Notch and Wnt signaling pathways genes in both adherent and spheres cells evaluated. Results: Sphere formation, clonogenic capacity, expression of Nestin protein, but not CD133 were increased in sphere cells in comparession to adherent cells. They also overexpressed β-catenin, cyclinD1, and c-Myc as Wnt down-stream genes, Notch1, HES1 as Notch down-stream genes, Nanog, and NESTIN as stem-related genes. Conclusion: These results suggest that sphere culture model could be a proper experimental method to separate cancer stem-like cells. Our data also support two important pathways are overactivated in melanoma cancer stem-like cells which must be considered in targeting therapy.
Jundishapur Scientific Medical Journal
Ahvaz Jundishapur University of Medical Sciences
2252-052X
16
v.
6
no.
2018
643
652
https://jsmj.ajums.ac.ir/article_57956_1297e3b6ec08139db86a97a024667ff6.pdf
dx.doi.org/10.22118/jsmj.2018.57956
Anthropometric Characteristics and Motor Fitness in Adolescent Boys with Relative and Non-relative
hasan
khalaji
Motor bihaviour and psychology D,Faculty of sport sciences,Arak university
author
Alireza
Bahrami
Associate Professor, of Behavioral Movement and Sports Psychology.Department of Exercise Behavior and Sports Psychology, Arak University of Medical Sciences, Arak, Iran.
author
Saeed
Housein Zadeh
Master Degree in the Field of Motor Behavior and Psychology.Department of Exercise Behavior and Sports Psychology, Arak University of Medical Sciences, Arak, Iran.
author
text
article
2018
per
Background and Objective: Relative marriages are one of the major factors in emergence of genetic disorders and disabilities, and it affects individual’s performance. Since marrages among relatives is common in our country, the study aimed to compare motor fitness and anthropometrical characteristics of teenager boys with nonrelative and relative parents. Subjects and Methods: In this Ex post facto research, 80 teenage boys of Arak city aged 14, 15, 16, 17 consisted of 20 people in non-relative groups and 20 people nonrelative groups were chosen from a targeted multi-stage cluster from Arak schools. The tool for gathering data, questionnaire of individual information, motor fitness test including: standing long jump and medicine ball throw, 36 meters running and ruler, 4×9 m shuttle run, BESS and test angel, Minnesota handing/eye and hand coordination. Anthropometric measuring tools consisted of tape measure, scale and stadiometer. For data analysis, Kolmogorov Smirnov, and for statistical operation, SPSS software version 17 was used. Results: Relative marriage of parents has a significant effect on moving readiness and anthropometric features of teenagers (p=/05). Conclusion: The findings of this study suggest that marrages among relatives has negative effects on moving readiness and anthropometric among teenage boys.
Jundishapur Scientific Medical Journal
Ahvaz Jundishapur University of Medical Sciences
2252-052X
16
v.
6
no.
2018
683
691
https://jsmj.ajums.ac.ir/article_58013_3549f5653758c06bbdaf68dadffa07a4.pdf
dx.doi.org/10.22118/jsmj.2018.58013
Comparison of the Effect of TRX and Traditional Resistance Training on Some Factors of Body Composition and Balance in Sedentary Men
Rouhollah
Ranjbar
Assistant Professor of Sport Physiology.Department of Sport Physiology, Faculty of Sport Sciences, Shahid Chamran University, Ahvaz, Iran.
author
Hesam
Hasanvand
Department of Exercise Physiology, Faculty of Sport Sciences, Shahid Chamran University, Ahvaz, Iran
author
Abdolhamid Habibi
Habibi
Professor of Sport Physiology.Department of Sport Physiology, Faculty of Sport Sciences, Shahid Chamran University, Ahvaz, Iran
author
Shahin
Goharpey
Assistant Professor of Physiotherapy.Center for Musculoskeletal Rehabilitation, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
author
text
article
2018
per
Background and Objective: Body composition and balance are always one of the main factors of health and quality of life among the people. The purpose of this study was to compare the effect of resistance training with TRX and Traditional on some factors of body composition and balance in Sedentary men. Subjects and Methods: InThis semi-experimental study, thirty Sedentary men (age = 29/33±3.23 and BMI = 26/03±1.24) participated and randomly divided to TRX group (n = 15) and traditional group (n = 15). Befor and after eight weeks of training, body composition (weight, BMI and percentage of body fat) and balance in three position (two legs, right leg and left leg) with Biodex stability system evaluated. Results: It was not observed any difference between groups in body composition (P>0/05); but the weight, BMI and percentage of body fat in traditional group and only percentage of body fat in TRX group significantly decreased (P<0/05). According to between group results, the overall balance of two legs and left leg and also ML balance of two legs, right leg and left leg in TRX group was significantly better than traditional group (P<0/05). While, ML balance of two legs and right leg had significant improvement only after TRX training (P<0/05), after traditional training, it was observed a significant decrease in overall and AP balance of right leg (P<0/05). Conclusion: It is consider that to compare with traditional resistance training, resistance training with TRX can be more effective in balance (not in body composition).
Jundishapur Scientific Medical Journal
Ahvaz Jundishapur University of Medical Sciences
2252-052X
16
v.
6
no.
2018
621
630
https://jsmj.ajums.ac.ir/article_58086_ce1a74be7b0fad3417bd67cb430181be.pdf
dx.doi.org/10.22118/jsmj.2018.58086